Patterson Lab Recognized by Neuromics for Primary Hippocampal Neuron Culture Techniques

Neuromics recently recognized The Patterson Lab at UC Davis for their outstanding culture techniques of primary hippocampal neurons:

http://neuromics.blogspot.com/2012/04/primary-hippocampal-neurons-performing.html 

Images of 67 day old e18 Primary Rat Hippocampal Neurons were provided by George Kenneth Todd!

Prior to staining, the cells were treated with Wnt5 for 2 min, then Wnt5 + Wnt3 for an additional 2 min during Calcium Imaging experiments. The cells were then fixed and stained for IP3R (green), Frizzled2 (blue), and B-Catenin (red), and the confocal images were captured at 10x. Notice the parallel neurites formation in image 3.